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Promega tfx20 transfection reagent
Tfx20 Transfection Reagent, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tfx20+transfection+reagent/tfx+50/pm19297606-41-36-39
Average 90 stars, based on 1 article reviews
tfx20 transfection reagent - by Bioz Stars, 2026-10
90/100 stars

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Related Articles

Transfection:

Article Title: Third strand-mediated psoralen-induced correction of the sickle cell mutation on a plasmid transfected into COS-7 cells.
Article Snippet: .. In the present work with the sickle cell target, we designed a complex delivery strand that binds to alternate strands of the target duplex in two different motifs (first G/T antiparallel and then pyrimidine/parallel), followed by a strand-invading element; in this case the psoralen is linked to the 30-terminus.18 Transfection conditions and plasmid expression COS-7 cells were transfected with DNA photoproducts using Tfx20 transfection reagent (Promega) and the Promega protocol for transfection of adherent cells. ..

Article Title: Targeted delivery of hepatitis C virus-specific short hairpin RNA in mouse liver using Sendai virosomes.
Article Snippet: .. Monolayers (70 % confluent) of Huh7 cells in 35 mm dishes were co-transfected with the shRNA plasmid construct and the HCV bicistronic plasmid DNAs or the CVB3 bicistronic construct along with a b-galactosidase (b-gal) construct using Tfx20 transfection reagent (Promega). .. Cells were harvested 24 h post-infection using passive lysis buffer (Promega) and Fluc and Rluc activities were measured using the Dual Luciferase assay system (Promega) in a TD 20/20 Luminometer (Turner Designs); the activities were normalized with respect to b-gal expression of the cells.

Plasmid Preparation:

Article Title: Third strand-mediated psoralen-induced correction of the sickle cell mutation on a plasmid transfected into COS-7 cells.
Article Snippet: .. In the present work with the sickle cell target, we designed a complex delivery strand that binds to alternate strands of the target duplex in two different motifs (first G/T antiparallel and then pyrimidine/parallel), followed by a strand-invading element; in this case the psoralen is linked to the 30-terminus.18 Transfection conditions and plasmid expression COS-7 cells were transfected with DNA photoproducts using Tfx20 transfection reagent (Promega) and the Promega protocol for transfection of adherent cells. ..

Article Title: Targeted delivery of hepatitis C virus-specific short hairpin RNA in mouse liver using Sendai virosomes.
Article Snippet: .. Monolayers (70 % confluent) of Huh7 cells in 35 mm dishes were co-transfected with the shRNA plasmid construct and the HCV bicistronic plasmid DNAs or the CVB3 bicistronic construct along with a b-galactosidase (b-gal) construct using Tfx20 transfection reagent (Promega). .. Cells were harvested 24 h post-infection using passive lysis buffer (Promega) and Fluc and Rluc activities were measured using the Dual Luciferase assay system (Promega) in a TD 20/20 Luminometer (Turner Designs); the activities were normalized with respect to b-gal expression of the cells.

Expressing:

Article Title: Third strand-mediated psoralen-induced correction of the sickle cell mutation on a plasmid transfected into COS-7 cells.
Article Snippet: .. In the present work with the sickle cell target, we designed a complex delivery strand that binds to alternate strands of the target duplex in two different motifs (first G/T antiparallel and then pyrimidine/parallel), followed by a strand-invading element; in this case the psoralen is linked to the 30-terminus.18 Transfection conditions and plasmid expression COS-7 cells were transfected with DNA photoproducts using Tfx20 transfection reagent (Promega) and the Promega protocol for transfection of adherent cells. ..

shRNA:

Article Title: Targeted delivery of hepatitis C virus-specific short hairpin RNA in mouse liver using Sendai virosomes.
Article Snippet: .. Monolayers (70 % confluent) of Huh7 cells in 35 mm dishes were co-transfected with the shRNA plasmid construct and the HCV bicistronic plasmid DNAs or the CVB3 bicistronic construct along with a b-galactosidase (b-gal) construct using Tfx20 transfection reagent (Promega). .. Cells were harvested 24 h post-infection using passive lysis buffer (Promega) and Fluc and Rluc activities were measured using the Dual Luciferase assay system (Promega) in a TD 20/20 Luminometer (Turner Designs); the activities were normalized with respect to b-gal expression of the cells.

Construct:

Article Title: Targeted delivery of hepatitis C virus-specific short hairpin RNA in mouse liver using Sendai virosomes.
Article Snippet: .. Monolayers (70 % confluent) of Huh7 cells in 35 mm dishes were co-transfected with the shRNA plasmid construct and the HCV bicistronic plasmid DNAs or the CVB3 bicistronic construct along with a b-galactosidase (b-gal) construct using Tfx20 transfection reagent (Promega). .. Cells were harvested 24 h post-infection using passive lysis buffer (Promega) and Fluc and Rluc activities were measured using the Dual Luciferase assay system (Promega) in a TD 20/20 Luminometer (Turner Designs); the activities were normalized with respect to b-gal expression of the cells.



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